Lectures and small group discussions focusing on topics of interest, varying from semester to semester.

Flow cytometry and cell sorting are crucial techniques in modern laboratories, helping to elucidate cell phenotypes and characterize the functions of highly heterogeneous cells and signals. Advances in hardware, reagents, and analytical software have significantly increased the number of measurable parameters and the speed of simultaneous measurements in single cells or events. Grasping the practical applications of these tools is vital for maximizing their potential and supporting research efforts. This course aims to introduce state-of-the-art Flow Cytometry technology commonly used in immunology research laboratories through detailed presentations and interactive exercises. Real examples and protocols for sample preparation, data collection, gating strategies for various cell types, data analysis, common problems, and practical applications of this technology will be demonstrated. It is designed for upper-division undergraduate students majoring in immunology and other biological sciences who plan to use flow cytometry in independent-study projects, research laboratories, or future employment. By the end of the course, students will have a comprehensive understanding of Flow Cytometry technology and its applications in immunology research.

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